culture conditions lihc cell lines (ATCC)

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ATCC culture conditions lihc cell lines
Culture Conditions Lihc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 30028 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/culture+conditions+cell+lines/Hep+G2/pm42210102-107-3-20
Average 99 stars, based on 30028 article reviews

culture conditions lihc cell lines - by Bioz Stars, 2026-10

99/100 stars

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Derivative Assay:

Article Title: MicroRNA miR-16-1 regulates CCNE1 (cyclin E1) gene expression in human cervical cancer cells
Article Snippet: .. Cell lines and culture conditions Cell lines derived from CC (C-33 A HPV-, SiHa HPV16+, CaSki HPV16+, HeLa HPV18+ cells) were obtained from the American Type Culture Collection (ATCC) and were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS), penicillin/streptomycin (50 μg/ml), 2 mM L-glutamine, 250 ng/mL fungizone, and maintained at 37°C in 5% CO 2 . .. The cells were used in transfection assays and total RNA isolation was carried out with TriPure isolation reagent (Roche, Indianapolis, IN) for the RT-qPCR assays. siRNA expression plasmids for human microRNA miR-16-1 DNA inserts of siRNAs specific for human microRNA hsa-miR-16-1 were designed using siRNA at Whitehead software (Applied Biosystems, Foster, CA) [ 19 ] and a genomic fragment spanning the miR-16-1 locus from human chromosome 13 was cloned in Apa I and Eco RI restriction sites in the pSilencer1.0-U6 siRNA expression plasmid (Applied Biosystems, Foster, CA), which contains the U6 RNA Pol-III promoter to generate small RNA transcripts, to generate the pSIMIR16-5P plasmid.

Cell Culture:

Modification:

Article Title: DNA replication-dependent induction of gene proximity by androgen.
Article Snippet: .. Cell lines and culture conditions Cell lines, including the SV40-immortalized prostate epithelial cell lines PNT1a and PNT2 (obtained from Norman Maitland and Colin Cooper, respectively) and prostate cancer cell lines LNCaP,DU145andVCaP(ATCC),were routinelycultured inDulbecco’s Modified Eagle’s Medium supplemented with 10% fetal bovine serum. .. Normal prostate epithelial cells (PrEC) (Lonza) were cultured in Clonetics PrEBM medium (Lonza).

other:

Article Title: LINC01614 Promotes Osteosarcoma Progression as ceRNA to Target SNX3 by Sponging hsa-miR-520a-3p
Article Snippet: Cell lines and culture conditions Cell lines (143B, MG63, HOS, V2OS, and Saos2) of Human OS and the osteoblast cell line (hFOB 1.19) of the human being were obtained from American Type Culture Collection.

Article Title: Prion-induced ferroptosis is facilitated by RAC3.
Article Snippet: Cell lines and culture conditions Cell lines used in this study: HT-1080 (ATCC #CCL-121), HEK-293T (ATCC #CRL-3216), HT22 (Sigma #SCC129).

Multiple Displacement Amplification:

Article Title: miR-27a regulates the sensitivity of breast cancer cells to cisplatin treatment via BAK-SMAC/DIABLO-XIAP axis.
Article Snippet: MicroRNA-27a (miR-27a) has been reported to be an onco-microRNA in multiple cancers promoting tumor growth and metastasis, but the role of miR-27a in regulating the cancer sensitivity to chemotherapy remains unknown.. In this study, upregulation of miR-27a was validated by real-time PCR analysis in breast cancer (BC) cell lines and samples of BC patients.. A negative correlation between miR-27a and bak was also observed in normal breast epithelial cell line MCF10A and BC cell lines, suggesting that the bak is the potential target of miR-27a. miR-27a could modulate the growth and metastasis of BC cells.

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